Electrophoresis is a laboratory technique used to separate and analyze molecules such as DNA, RNA and proteins based on their size and electrical charge. It involves placing the molecules in a gel or matrix and passing an electric current through it, allowing smaller molecules to move faster than larger ones. This process helps in determining the size of molecules in a sample and comparing them to standards of known sizes.
Electrophoresis is a popular technique used in the life sciences to separate molecules by size, density and purity. Different methods are used depending on the type and size of the molecules being analyzed. It requires a source of electrical charge, a carrier medium and buffer solution. Electrophoresis is commonly used to identify source DNA in paternity testing and forensics.
The word Electrophoresis derives from ancient Greek and refers to the movement of dispersed particles relative to a liquid under the influence of a spatially uniform electric field. It roughly describes the movement of particles in a gel or liquid in a relatively uniform electric field.
What is Separated Through Electrophoresis?
Electrophoresis is used to separate molecules such as DNA, RNA, proteins, nucleic acids, plasmids and fragments of these macromolecules.
What is the Purpose of Electrophoresis?
The purpose of electrophoresis is to analyze and identify the size, density and purity of molecules so that they can be studied and compared to known standards.
What Types of Electrophoresis are There?
There are several types of electrophoresis such as agarose gel electrophoresis, polyacrylamide gel electrophoresis, pulsed-field gel electrophoresis and capillary electrophoresis, each with their specific applications and methods.
Overall, electrophoresis is a vital laboratory technique used in the study of biomolecules, allowing for detailed analysis of DNA, RNA and proteins.